nrg1 levels  (Boster Bio)

Journal: BMC Psychiatry

Article Title: Antipsychotic drugs increase Neuregulin1β1 serum levels in first-episode drug-naïve patients and chronic schizophrenia with suggestions for improving the treatment of psychotic symptoms

doi: 10.1186/s12888-022-03856-9

Figure Lengend Snippet: NRG1β1 levels in the patient and control groups before and after antipsychotic drug treatment

Article Snippet: Serum NRG1β1 levels were tested using sandwich enzyme-linked immunosorbent assays (ELISAs) in accordance with the manufacturer’s instructions (DY377; R&D Systems, Minneapolis, MN, USA).

Techniques:

Journal: BMC Psychiatry

Article Title: Antipsychotic drugs increase Neuregulin1β1 serum levels in first-episode drug-naïve patients and chronic schizophrenia with suggestions for improving the treatment of psychotic symptoms

doi: 10.1186/s12888-022-03856-9

Figure Lengend Snippet: NRG1β1 levels in the responder and nonresponder groups before and after antipsychotic drug treatment

Article Snippet: Serum NRG1β1 levels were tested using sandwich enzyme-linked immunosorbent assays (ELISAs) in accordance with the manufacturer’s instructions (DY377; R&D Systems, Minneapolis, MN, USA).

Techniques:

Journal: bioRxiv

Article Title: Early neuroadaptations to an obesogenic diet identify the schizophrenia-related ErbB4 receptor in obesity-induced hippocampal abnormalities

doi: 10.1101/2021.06.30.450398

Figure Lengend Snippet: (A) Study 1 timeline and study groups. (B) Study 1 timeline of behavioral procedures, MRI, and histology. (C) Study 2 timeline of molecular analyses. Abbreviations: CD , control diet; EPM , elevated plus maze; FPS , fear-potentiated startle; MRI , magnetic resonance imaging; NRG1 , neuregulin-1; OFT , open field test; PND , postnatal day; VEH , vehicle; WD , Western diet.

Article Snippet: NRG1 levels were determined using the SpectraMax i3X plate reader (Molecular Devices, Sunnyvale, CA USA) as the mean absorbance at 450nm with correction at 540 nm using a standard curve of samples ranging from 0 to 4,000 pg/mL.

Techniques: Magnetic Resonance Imaging, Western Blot

Journal: bioRxiv

Article Title: Early neuroadaptations to an obesogenic diet identify the schizophrenia-related ErbB4 receptor in obesity-induced hippocampal abnormalities

doi: 10.1101/2021.06.30.450398

Figure Lengend Snippet: (A-B) Total hippocampal volume (both left and right hippocampi combined) were affected by the treatment [ F (1, 19) = 9.48, p = .006]. Subchronic NRG1 administration during adolescence resulted in significant total hippocampal volume reduction (CDV vs. CDN, p = .003). (C-D) Hippocampal subfield segmentation showed a significant interaction [ F (1, 19) = 6.44, p = .020] effects on CA1 region. WDV rats had a significantly smaller CA1 volume than CDV rats ( p = .015). Sample size = 6 rats / group.

Article Snippet: NRG1 levels were determined using the SpectraMax i3X plate reader (Molecular Devices, Sunnyvale, CA USA) as the mean absorbance at 450nm with correction at 540 nm using a standard curve of samples ranging from 0 to 4,000 pg/mL.

Techniques:

Journal: bioRxiv

Article Title: Early neuroadaptations to an obesogenic diet identify the schizophrenia-related ErbB4 receptor in obesity-induced hippocampal abnormalities

doi: 10.1101/2021.06.30.450398

Figure Lengend Snippet: Full-focused composite 40x images were acquired from twelve (12) rats. Hippocampal dorsal and ventral CA1 areas were scanned to obtain at least three (3) images per region of interest per hemisphere covering a field of view area of 1.2 mm. Each acquired image was saved as a TIFF file and included at least six (6) Iba-1 + cells. (A) Representative microglia morphologies from hippocampal dorsal and ventral CA1 regions in each study group. (B) Evaluation of microglial density as a measure of cell size based on area demonstrated that the WD significantly decreased microglial density in the right CA1 subfield (diet: [ F (1,8) = 6.82, p < .031]). (C) Lacunarity was significantly decreased in the right CA1 subfield of rats that consumed an obesogenic WD ( diet: [ F (1,8) = 12.09, p < .008]). The span ratio was significantly increased in the right CA1 region of rats receiving the exogenous NRG1 ( treatment: [ F (1,8) = 15.43, p < .004]). NRG1 administration reduced circularity in the right CA1 region ( treatment: [ F (1,8) = 9.67, p < .014]). Sample size = 3 rats / group.

Article Snippet: NRG1 levels were determined using the SpectraMax i3X plate reader (Molecular Devices, Sunnyvale, CA USA) as the mean absorbance at 450nm with correction at 540 nm using a standard curve of samples ranging from 0 to 4,000 pg/mL.

Techniques:

Journal: bioRxiv

Article Title: Early neuroadaptations to an obesogenic diet identify the schizophrenia-related ErbB4 receptor in obesity-induced hippocampal abnormalities

doi: 10.1101/2021.06.30.450398

Figure Lengend Snippet: Bead-based flow cytometry immunoassay was used to evaluate cytokine levels. (A-D) The WD led to a significant increase in cytokines in the hippocampus: IL1-α ( p = .010), TNF-α ( p = .006), IL-10 ( p = .027), and IL-6 ( p = .019). (E-H) NRG1 administration significantly reduced IL-33 ( p = .041), GM-CSF ( p = .021), CCL-2 ( p = .037), and IFN-γ ( p = .031). includes the detailed statistics of the cytokines listed in . Sample size = 5-6 rats / group.

Article Snippet: NRG1 levels were determined using the SpectraMax i3X plate reader (Molecular Devices, Sunnyvale, CA USA) as the mean absorbance at 450nm with correction at 540 nm using a standard curve of samples ranging from 0 to 4,000 pg/mL.

Techniques: Flow Cytometry

Journal: bioRxiv

Article Title: Early neuroadaptations to an obesogenic diet identify the schizophrenia-related ErbB4 receptor in obesity-induced hippocampal abnormalities

doi: 10.1101/2021.06.30.450398

Figure Lengend Snippet: (A) Obesogenic WD decreased total ErbB4 protein levels in the hippocampus (diet [ F (1, 16) = 7.34, p = .016]). WDN rats exhibited significantly lower ErbB4 protein levels when compared to CDV rats ( p = .009). (B) Phosphorylation of hippocampal ErbB4 protein levels was affected by the treatment [ F (1, 18) = 5.82, p = .027] and diet [ F (1,18) = 7.36, p = .014]. ErbB4 hyperphosphorylation was particularly evident in WDN rats relative to the CDV group ( p = .012). (C) TACE/ADAM17 protein levels in the hippocampus were affected by the treatment [ F (1, 17) = 8.93, p = .008] and diet [ F (1, 17) = 11.03, p = .004]. WDN rats showed increased TACE/ADAM17 protein levels when compared to CDV rats ( p = .001). (D) NRG1 administration showed a significant decrease for PSD-95 protein levels in the hippocampus (treatment [ F (1, 18) = 4.47, p = .048]). Sample size = 5-6 rats / group.

Article Snippet: NRG1 levels were determined using the SpectraMax i3X plate reader (Molecular Devices, Sunnyvale, CA USA) as the mean absorbance at 450nm with correction at 540 nm using a standard curve of samples ranging from 0 to 4,000 pg/mL.

Techniques: